Antibodies to Epstein-Barr Virus Proteins

anti-EBV Nuclear Antigen-1 (EBNA-1)

Mouse monoclonal IgG2

Recommended for Western blotting, immunoprecipitation, immunohistochemistry

Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)

Epitope corresponding to aminoacids 610-640 mapping at the carboxyl terminus of EBNA-1 protein

Using immunostaining the antigen is detected in the nucleus by this antibody

anti-EBV Early Antigen-R (EA-R) Bcl-2 homologue

Mouse monoclonal IgG1

Recommended for Western blotting, immunoprecipitation, immunohistochemistry

Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)

Using immunostaining the antigen is detected in the cytoplasm by this antibody (staining pattern changes with time in the life cycle of the virus)
Click on the pictures

anti-EBV interleukin-10 (v-IL-10) IL-10 homologue

Mouse monoclonal IgG1

Recommended for Western blotting, immunoprecipitation, immunohistochemistry

Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)

anti-EBV Early Antigen-R (EA-R) ribonucleotide reductase large subunit

Mouse monoclonal IgG2

Recommended for Western blotting, immunoprecipitation, immunohistochemistry

Ribonuclease activity is not inhibited by this antibody in in vitro assay

Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)

Using immunostaining the antigen is detected in the cytoplasm by this antibody (staining pattern changes with time in the life cycle of the virus see also gallery)
Click on the pictures

anti-EBV Early Antigen-R (EA-R) ribonucleotide reductase large subunit

Mouse monoclonal IgG1

Recommended for immunoprecipitation, immunohistochemistry

Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)

This antibody does not recognize the antigen on Westernblotts

Ribonuclease activity is inhibited by this antibody in in vitro assay

Recognized epitope is different from the previous antibody
Using immunostaining the antigen is detected in the cytoplasm by this antibody

anti-EBV Early Antigen-R (EA-R) ribonucleotide reductase large subunit

Mouse monoclonal IgG1

Recommended for immunoprecipitation, immunohistochemistry

Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)

This antibody does not recognize the antigen on Westernblotts

Recognized epitope is different from the previous two antibodies
Using immunostaining the antigen is detected in the cytoplasm by this antibody
Click on the picture

anti-EBV Early Antigen-D (EA-D) p47-53

Mouse monoclonal IgG2

Recommended for Western blotting, immunoprecipitation, immunohistochemistry

Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)

Using immunostaining the antigen is detected in the nucleus and cytoplasm by this antibody

Epitope is at the carboxyl terminus of the protein

Click on the pictures

anti-EBV Early Antigen-D (EA-D) p47-53

Mouse monoclonal IgG2

Recommended for Western blotting, immunoprecipitation, immunohistochemistry

Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)

Using immunostaining the antigen is detected in the nucleus and cytoplasm by this antibody (occasionally the antigen can diffuse out from the nucleus and the staining is mainly cytoplasmic {last two pictures. This happens if the drying of the cells on the slides was to slow)

Epitope is at the middle part of the protein

 

Click on the pictures

anti-EBV Viral Capsid Antigen (VCA) p 140

Mouse monoclonal IgG2

Recommended for immunoprecipitation, immunohistochemistry

Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)

This antibody does not recognize the antigen on Westernblotts

Using immunostaining the antigen is detected in the nucleus by this antibody

 

Click on the pictures

anti-EBV Viral Capsid Antigen (VCA) p 140

Mouse monoclonal IgG2

Recommended for immunoprecipitation, immunohistochemistry

Stains formalin fixed paraffin-embedded tissue sections after

antigen retrieval (see recommended protocols)

This antibody recognizes the antigen on Westernblotts but the signal is weak

Recognized epitope is different from the previous antibody
Using immunostaining the antigen is detected in the nucleus by this antibody

 

Click on the pictures

anti-EBV Envelope Protein (VCA) gp 110-120

Mouse monoclonal IgG2

Recommended for Western blotting, immunoprecipitation, immunohistochemistry

Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)

Using immunostaining the antigen is detected in the cytoplasm by this antibody

 

Click on the pictures

anti-EBV Envelope Protein (VCA) GP 110-120

Mouse monoclonal IgG2

Recommended for Western blotting, immunoprecipitation, immunohistochemistry

Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)

Recognized epitope is different from the previous antibody
Using immunostaining the antigen is detected in the cytoplasm by this antibody

 

Click on the pictures

anti-EBV Envelope Protein (VCA) GP 110-120

Mouse monoclonal IgG2

Recommended for Western blotting, immunoprecipitation, immunohistochemistry

Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)

Recognized epitope is different from the previous two antibodies
Using immunostaining the antigen is detected in the cytoplasm by this antibody

anti-EBV Membrane Antigen (MA) gp220-350

Mouse monoclonal IgG1

Recommended for immunoprecipitation, immunohistochemistry

Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)

This antibody does not recognize the antigen on Westernblotts

Using immunostaining the antigen is detected in the cytoplasm and on the membrane by this antibody

 

Click on the pictures

anti-EBV Membrane Antigen (MA) gp350

Mouse monoclonal IgG1

Recommended for immunoprecipitation, immunohistochemistry

Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)

This antibody does not recognize the antigen on Westernblotts

Recognized epitope is different from the previous antibody
Recognizes only A-type EBV antigen.

Using immunostaining the antigen is detected in the cytoplasm and on the membrane by this antibody

 

Click on the picture

anti-EBV Membrane Antigen (MA) gp350

Mouse monoclonal IgG1

Recommended for immunoprecipitation, immunohistochemistry

Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)

This antibody recognizes the antigen on Westernblotts

Recognized epitope is different from the previous two antibodies
Recognizes unglycosylated and glycosylated forms of the antigen

Using immunostaining the antigen is detected in the cytoplasm by this antibody

Antibodies to Human Herpesvirus 6

anti-HHV-6 Viral Capsid Antigen (VCA) p 140 (H-JG-16)

Mouse monoclonal IgG1

Recommended for immunoprecipitation, immunohistochemistry

Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)

This antibody does not recognize the antigen on Westernblotts

Using immunostaining the antigen is detected in the nucleus by this antibody

anti-HHV-6 Viral Capsid Antigen (VCA) p 140 (H-JG-20)

Mouse monoclonal IgG1

Recommended for immunoprecipitation, immunohistochemistry

Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)

This antibody does not recognize the antigen on Westernblotts

Using immunostaining the antigen is detected in the nucleus by this antibody
Recognized epitope is different from the previous antibody

anti-HHV-6 Envelope Antigen (VCA) GP 85-110 (H-AR-1)

Mouse monoclonal IgG2

Recommended for immunoprecipitation, immunohistochemistry and westernblotting

Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)

Using immunostaining the antigen is detected in the cytoplasm and on the membrane by this antibody

Neutralizes virus infection in vitro

anti-HHV-6 Envelope Antigen (VCA) GP 85-110 (H-AR-2)

Mouse monoclonal IgG2

Recommended for immunoprecipitation, immunohistochemistry and westernblotting

Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)

Using immunostaining the antigen is detected in the cytoplasm and on the membrane by this antibody

Neutralizes virus infection in vitro

Recognized epitope is different from the previous antibody

Mouse monoclonal IgG2

Recommended for immunoprecipitation, immunohistochemistry and westernblotting

Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)

Using immunostaining the antigen is detected in the cytoplasm and on the membrane by this antibody

Neutralizes virus infection in vitro

Recognized epitope is different from the previous two antibodies

Mouse monoclonal IgG1

Recommended for immunoprecipitation, immunohistochemistry and westernblotting

Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)

Using immunostaining the antigen is detected in the cytoplasm and on the membrane by this antibody

Neutralizes virus infection in vitro

Recognized epitope is different from the previous three antibodies

anti-HHV-6 Membrane Antigen (MA) GP 90 (H-AR-8)

Mouse monoclonal IgG2

Recommended for immunoprecipitation, immunohistochemistry and westernblotting

Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)

Using immunostaining the antigen is detected in the cytoplasm and on the membrane by this antibody

Partially neutralizes virus infection in vitro

Antibodies to Human Herpesvirus 7

anti-HHV-7 Envelope Antigen (VCA) GP 90-110

Mouse monoclonal IgG2

Recommended for immunoprecipitation, immunohistochemistry and westernblotting

Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)

Using immunostaining the antigen is detected in the cytoplasm and on the membrane by this antibody

Neutralizes virus infection in vitro

anti-HHV-7 Envelope Antigen (VCA) GP 90-110

Mouse monoclonal IgG2

Recommended for immunoprecipitation, immunohistochemistry and westernblotting

Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)

Using immunostaining the antigen is detected in the cytoplasm and on the membrane by this antibody

Neutralizes virus infection in vitro

Weak cross-reactivity with HHV-6 envelop protein

Recognized epitope is different from the previous antibody

Antibodies to Cytomegalovirus

anti-CMV tegument protein (VCA) p68

Mouse monoclonal IgG2

Recommended for immunoprecipitation, immunohistochemistry and westernblotting

Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)

Using immunostaining the antigen is detected in the nucleus and cytoplasm by this antibody

anti-CMV Immediately early antigen (IEA) p72

Mouse monoclonal IgG2

Recommended for immunoprecipitation, immunohistochemistry and westernblotting

Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)

Using immunostaining the antigen is detected in the nucleus by this antibody

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