Make your own free website on Tripod.com

Antibodies to Epstein-Barr Virus Proteins

anti-EBV Nuclear Antigen-1 (EBNA-1)
Mouse monoclonal IgG2
Recommended for Western blotting, immunoprecipitation, immunohistochemistry
Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)
Epitope corresponding to aminoacids 610-640 mapping at the carboxyl terminus of EBNA-1 protein
Using immunostaining the antigen is detected in the nucleus by this antibody


anti-EBV Early Antigen-R (EA-R) Bcl-2 homologue
Mouse monoclonal IgG1
Recommended for Western blotting, immunoprecipitation, immunohistochemistry
Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)
Using immunostaining the antigen is detected in the cytoplasm by this antibody (staining pattern changes with time in the life cycle of the virus)
Click on the pictures

anti-EBV interleukin-10 (v-IL-10) IL-10 homologue
Mouse monoclonal IgG1
Recommended for Western blotting, immunoprecipitation, immunohistochemistry
Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)


anti-EBV Early Antigen-R (EA-R) ribonucleotide reductase large subunit
Mouse monoclonal IgG2
Recommended for Western blotting, immunoprecipitation, immunohistochemistry
Ribonuclease activity is not inhibited by this antibody in in vitro assay
Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)
Using immunostaining the antigen is detected in the cytoplasm by this antibody (staining pattern changes with time in the life cycle of the virus see also gallery)
Click on the pictures

anti-EBV Early Antigen-R (EA-R) ribonucleotide reductase large subunit
Mouse monoclonal IgG1
Recommended for immunoprecipitation, immunohistochemistry
Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)
This antibody does not recognize the antigen on Westernblotts
Ribonuclease activity is inhibited by this antibody in in vitro assay
Recognized epitope is different from the previous antibody
Using immunostaining the antigen is detected in the cytoplasm by this antibody


anti-EBV Early Antigen-R (EA-R) ribonucleotide reductase large subunit
Mouse monoclonal IgG1
Recommended for immunoprecipitation, immunohistochemistry
Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)
This antibody does not recognize the antigen on Westernblotts
Recognized epitope is different from the previous two antibodies
Using immunostaining the antigen is detected in the cytoplasm by this antibody
Click on the picture

anti-EBV Early Antigen-D (EA-D) p47-53
Mouse monoclonal IgG2
Recommended for Western blotting, immunoprecipitation, immunohistochemistry
Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)
Using immunostaining the antigen is detected in the nucleus and cytoplasm by this antibody
Epitope is at the carboxyl terminus of the protein

Click on the pictures

anti-EBV Early Antigen-D (EA-D) p47-53
Mouse monoclonal IgG2
Recommended for Western blotting, immunoprecipitation, immunohistochemistry
Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)
Using immunostaining the antigen is detected in the nucleus and cytoplasm by this antibody (occasionally the antigen can diffuse out from the nucleus and the staining is mainly cytoplasmic {last two pictures. This happens if the drying of the cells on the slides was to slow)
Epitope is at the middle part of the protein
 
Click on the pictures


anti-EBV Viral Capsid Antigen (VCA) p 140
Mouse monoclonal IgG2
Recommended for immunoprecipitation, immunohistochemistry
Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)
This antibody does not recognize the antigen on Westernblotts
Using immunostaining the antigen is detected in the nucleus by this antibody
 
Click on the pictures


anti-EBV Viral Capsid Antigen (VCA) p 140
Mouse monoclonal IgG2
Recommended for immunoprecipitation, immunohistochemistry
Stains formalin fixed paraffin-embedded tissue sections after
antigen retrieval (see recommended protocols)
This antibody recognizes the antigen on Westernblotts but the signal is weak
Recognized epitope is different from the previous antibody
Using immunostaining the antigen is detected in the nucleus by this antibody
 
Click on the pictures


anti-EBV Envelope Protein (VCA) gp 110-120
Mouse monoclonal IgG2
Recommended for Western blotting, immunoprecipitation, immunohistochemistry
Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)
Using immunostaining the antigen is detected in the cytoplasm by this antibody
 
Click on the pictures


anti-EBV Envelope Protein (VCA) GP 110-120
Mouse monoclonal IgG2
Recommended for Western blotting, immunoprecipitation, immunohistochemistry
Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)
Recognized epitope is different from the previous antibody
Using immunostaining the antigen is detected in the cytoplasm by this antibody
 
Click on the pictures


anti-EBV Envelope Protein (VCA) GP 110-120
Mouse monoclonal IgG2
Recommended for Western blotting, immunoprecipitation, immunohistochemistry
Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)
Recognized epitope is different from the previous two antibodies
Using immunostaining the antigen is detected in the cytoplasm by this antibody


anti-EBV Membrane Antigen (MA) gp220-350
Mouse monoclonal IgG1
Recommended for immunoprecipitation, immunohistochemistry
Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)
This antibody does not recognize the antigen on Westernblotts
Using immunostaining the antigen is detected in the cytoplasm and on the membrane by this antibody
 
Click on the pictures


anti-EBV Membrane Antigen (MA) gp350
Mouse monoclonal IgG1
Recommended for immunoprecipitation, immunohistochemistry
Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)
This antibody does not recognize the antigen on Westernblotts
Recognized epitope is different from the previous antibody
Recognizes only A-type EBV antigen.
Using immunostaining the antigen is detected in the cytoplasm and on the membrane by this antibody
 
Click on the picture


anti-EBV Membrane Antigen (MA) gp350
Mouse monoclonal IgG1
Recommended for immunoprecipitation, immunohistochemistry
Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)
This antibody recognizes the antigen on Westernblotts
Recognized epitope is different from the previous two antibodies
Recognizes unglycosylated and glycosylated forms of the antigen
Using immunostaining the antigen is detected in the cytoplasm by this antibody

Antibodies to Human Herpesvirus 6

anti-HHV-6 Viral Capsid Antigen (VCA) p 140 (H-JG-16)
Mouse monoclonal IgG1
Recommended for immunoprecipitation, immunohistochemistry
Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)
This antibody does not recognize the antigen on Westernblotts
Using immunostaining the antigen is detected in the nucleus by this antibody


anti-HHV-6 Viral Capsid Antigen (VCA) p 140 (H-JG-20)
Mouse monoclonal IgG1
Recommended for immunoprecipitation, immunohistochemistry
Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)
This antibody does not recognize the antigen on Westernblotts
Using immunostaining the antigen is detected in the nucleus by this antibody
Recognized epitope is different from the previous antibody


anti-HHV-6 Envelope Antigen (VCA) GP 85-110 (H-AR-1)
Mouse monoclonal IgG2
Recommended for immunoprecipitation, immunohistochemistry and westernblotting
Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)
Using immunostaining the antigen is detected in the cytoplasm and on the membrane by this antibody
Neutralizes virus infection in vitro


anti-HHV-6 Envelope Antigen (VCA) GP 85-110 (H-AR-2)
Mouse monoclonal IgG2
Recommended for immunoprecipitation, immunohistochemistry and westernblotting
Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)
Using immunostaining the antigen is detected in the cytoplasm and on the membrane by this antibody
Neutralizes virus infection in vitro
Recognized epitope is different from the previous antibody

anti-HHV-6 Envelope Antigen (VCA) GP 85-110 (H-AR-4)
Mouse monoclonal IgG2
Recommended for immunoprecipitation, immunohistochemistry and westernblotting
Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)
Using immunostaining the antigen is detected in the cytoplasm and on the membrane by this antibody
Neutralizes virus infection in vitro
Recognized epitope is different from the previous two antibodies

anti-HHV-6 Envelope Antigen (VCA) GP 85-110 (H-AR-6)
Mouse monoclonal IgG1
Recommended for immunoprecipitation, immunohistochemistry and westernblotting
Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)
Using immunostaining the antigen is detected in the cytoplasm and on the membrane by this antibody
Neutralizes virus infection in vitro
Recognized epitope is different from the previous three antibodies


anti-HHV-6 Membrane Antigen (MA) GP 90 (H-AR-8)
Mouse monoclonal IgG2
Recommended for immunoprecipitation, immunohistochemistry and westernblotting
Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)
Using immunostaining the antigen is detected in the cytoplasm and on the membrane by this antibody
Partially neutralizes virus infection in vitro


Antibodies to Human Herpesvirus 7

anti-HHV-7 Envelope Antigen (VCA) GP 90-110
Mouse monoclonal IgG2
Recommended for immunoprecipitation, immunohistochemistry and westernblotting
Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)
Using immunostaining the antigen is detected in the cytoplasm and on the membrane by this antibody
Neutralizes virus infection in vitro

anti-HHV-7 Envelope Antigen (VCA) GP 90-110
Mouse monoclonal IgG2
Recommended for immunoprecipitation, immunohistochemistry and westernblotting
Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)
Using immunostaining the antigen is detected in the cytoplasm and on the membrane by this antibody
Neutralizes virus infection in vitro
Weak cross-reactivity with HHV-6 envelop protein
Recognized epitope is different from the previous antibody

Antibodies to Cytomegalovirus

anti-CMV tegument protein (VCA) p68
Mouse monoclonal IgG2
Recommended for immunoprecipitation, immunohistochemistry and westernblotting
Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)
Using immunostaining the antigen is detected in the nucleus and cytoplasm by this antibody

anti-CMV Immediately early antigen (IEA) p72
Mouse monoclonal IgG2
Recommended for immunoprecipitation, immunohistochemistry and westernblotting
Stains formalin fixed paraffin-embedded tissue sections after antigen retrieval (see recommended protocols)
Using immunostaining the antigen is detected in the nucleus by this antibody


Back to Janos Luka's Home Page



Revised: June 5, 2000